The ProNet™ Process

Our protein interaction experts will recommend the optimal number of bait constructs for the protein of your choice

• We recommend multiple baits for each protein, using small modular domains (100- 300 amino acids) for best search results

We can amplify your bait from cDNA libraries or a furnished DNA template. Once the bait construct has been made:

• It undergoes rigorous sequence verification
  • Correct reading frame with no mutations every time.
• Bait construct auto-activation testing is done to eliminate possible false positive readings

• Our reporter system is based on 3 reporter genes with distinct promoter regions
  • ADE2 auxotrophic marker gene
  • HIS3 auxotrophic marker gene
  • Beta-GAL enzymatic marker gene
• The odds of spuriously activating all 3 reporter genes is extremely low
• Liquid mating against three or more libraries per bait and allows for searches testing 100 million possible interactions
• Our standard ProNet package allows searches of three individual activation domain libraries of your choice, to ensure maximal interaction coverage

• A concise data summary slide
• An Excel spreadsheet of raw data
• SIF formatted files for use in Cytoscape