ProNet™ Discoveries

ProNet is all about leading your laboratory in new directions

 

In conjunction with University of Utah researcher, Dr. Wesley Sundquist, the ProNet team has helped redefine the biology surrounding HIV cellular egress. With information provided by the ProNet database, Dr. Sundquist embarked on a series of discoveries that not only showed how HIV hijacks the cellular pathways of vesicular trafficking for it's own replication, but was also able to publish this research in high impact journals such as Cell, Nature & EMBO.This innovative research resulted in several coveted NIH grants including 2 multimillion dollar RO1 grants and a five-year, $19.2 million National Institutes of Health (NIH) center of excellence grant to establish an HIV research hub for the study of HIV structural biology.

Myrexis' industrial scale Yeast Two-Hybrid platform is ideal for whole proteome analysis of microbial organisms. Indeed, Myrexis was selected by the National Institute of Allergy and Infectious Disease to carry out a comprehensive Yeast Two-Hybrid analysis of pathogen-host interactions for several Category A pathogens. This 14.2 Million dollar project entitled, "Identifying Targets for Therapeutic Interventions Using Proteomics Technology", analyzed the proteomes of several pathogenic organisms including Variola major, Vaccinia, Bacillus anthracis, Yersinia pestis and Fransicella tularensis.

Further proteomic analysis of our Yeast Two-Hybrid Vaccinia/host interactions (Zhang et al., 2009) demonstrated that 63% of the interactions investigated were confirmed by GST pulldown assays. Whole Proteome analysis by ProNet can lead to innovation in virtually any area of biology!

Let ProNet help jumpstart the type of edgy, innovative science that can return big dividends for your laboratory as well!

 

Virology Examples

J. E. Garrus, U. K. Von Schwedler, O. W. Pornillos, S.G. Morham, K. H. Zavitz, H. E. Wang, D. A. Wettstein, K. M. Stray, M. Cote, R. L. Rich, D. G. Myszka and W. I. Sundquist. (2001) Tsg101 and the vacuolar protein sorting pathway are essential for HIV-1 budding. Cell 107, 55-65. View abstract

U.K. von Schwedler, M. Stuchell, B. Müller, D. Ward, H.Y. Chung, E. Morita, H.E. Wang, T. Davis, G.P. He, D.M. Cimbora, A. Scott, H.G. Kräusslich, J. Kaplan, S.G. Morham, and W.I. Sundquist. The Protein Network of HIV Budding. (2003) Cell 114, 701-713.View abstract

M.D. Stuchell, J.E. Garrus, B. Muller, K.M. Stray, S. Ghaffarian, R. McKinnon, H.G. Krausslich, S.G. Morham and W.I. Sundquist. The human endosomal complex required for transport I (ESCRT-I) and its role in HIV-1 budding. (2004) J Biol Chem. 279, 36059-36071. View abstract

E. Morita, V. Sandrin, H.Y. Chung, S.G. Morham, S.P. Gygi, C.K. Rodesch and W.I. Sundquist. Human ESCRT and ALIX proteins interact with proteins of the midbody and function in cytokinesis. (2007) EMBO J. 26, 4215-4227. View abstract

M.D. Stuchell-Brereton, J.J. Skalicky, C. Kieffer, M.A. Karren, S. Ghaffarian, W.I. Sundquist. ESCRT-III recognition by VPS4 ATPases. (2007) Nature 449, 740-744. View abstract

M. Bajorek, E. Morita, J.J. Skalicky, S.G. Morham, M. Babst and W.I. Sundquist. Biochemical Analyses of Human IST1 and Its Function in Cytokinesis. (2009) Mol Biol Cell. 20, 1360-1373. View abstract

L Zhang, NY Villa, MM Rahman, S Smallwood, D Shattuck, C Neff, M Dufford, JS Lanchbury, J Labaer, G McFadden. Analysis of vaccinia virus-host protein-protein interactions: validations of yeast two-hybrid screenings. (2009) J Proteome Res. 8, 4311-4318. View abstract